Device

Part:BBa_K1919305:Design

Designed by: Shaobo Yang   Group: iGEM16_SCU-China   (2016-10-14)


livM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 761
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 437


Design Notes

The restriction sites are eliminated by mutation.


Source

The gene comes from genome of E.coli DH5a. We eliminated restriction site in coding region and send it to be synthsized by company.

References